TLE1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98893

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TLE1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TLE1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TLE1 Antibody

Western Blot: TLE1 Antibody [NBP2-98893]

Western Blot: TLE1 Antibody [NBP2-98893]

Western Blot: TLE1 Antibody [NBP2-98893] - Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:83 kDaObserved band size:78 kDa(We are unsure as to the identity of these extra bands.)
Immunocytochemistry/ Immunofluorescence: TLE1 Antibody [NBP2-98893]

Immunocytochemistry/ Immunofluorescence: TLE1 Antibody [NBP2-98893]

Immunocytochemistry/Immunofluorescence: TLE1 Antibody [NBP2-98893] - Immunofluorescence staining of TLE1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human TLE1 polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-98893]

Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-98893]

Immunohistochemistry-Paraffin: TLE1 Antibody [NBP2-98893] - Staining of human TLE1 in human colon carcinoma with rabbit polyclonal antibody (1:5000).
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Applications for TLE1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1500-1:50000

Immunohistochemistry-Paraffin

1:2500-1:10000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLE1

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG

Long Name

Transducin-like Enhancer of Split 1

Alternate Names

GRG1

Gene Symbol

TLE1

Additional TLE1 Products

Product Documents for TLE1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLE1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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