TLE3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30646

Novus Biologicals, part of Bio-Techne
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NBP2-30646
NBP2-30646-25ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TLE3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TLE3 Antibody

Western Blot: TLE3 Antibody [NBP2-30646]

Western Blot: TLE3 Antibody [NBP2-30646]

Western Blot: TLE3 Antibody [NBP2-30646] - Analysis in HEK293 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-TLE3 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH
Immunocytochemistry/ Immunofluorescence: TLE3 Antibody [NBP2-30646]

Immunocytochemistry/ Immunofluorescence: TLE3 Antibody [NBP2-30646]

Immunocytochemistry/Immunofluorescence: TLE3 Antibody [NBP2-30646] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646]

Immunohistochemistry-Paraffin: TLE3 Antibody [NBP2-30646] - Staining of human uterine cervix shows moderate to strong nuclear positivity in squamous epithelial cells.
View 3 more images

Applications for TLE3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLE3

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES

Long Name

Transducin-like Enhancer of Split 3

Alternate Names

ESG3, Grg3

Gene Symbol

TLE3

UniProt

Q04726

Additional TLE3 Products

Product Documents for TLE3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TLE3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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