TLE4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15140

Novus Biologicals, part of Bio-Techne
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NBP2-15140
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Chicken (100%), Mouse (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TLE4 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TLE4 Antibody

Western Blot: TLE4 Antibody [NBP2-15140]

Western Blot: TLE4 Antibody [NBP2-15140]

Western Blot: TLE4 Antibody [NBP2-15140] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TLE4 Antibody [NBP2-15140]

Immunocytochemistry/ Immunofluorescence: TLE4 Antibody [NBP2-15140]

Immunocytochemistry/Immunofluorescence: TLE4 Antibody [NBP2-15140] - DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE4 stained by TLE4 antibody [C1C3] diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140]

Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140]

Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.

Applications for TLE4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TLE4

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES.

Alternate Names

BCE1, BCE-1, E(spI), ESG4, homolog of Drosophila E(sp1), KIAA1261, Protein BCE-1, transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila), transducin-like enhancer protein 4

Gene Symbol

TLE4

UniProt

Q04727 (Human)

Additional TLE4 Products

Product Documents for TLE4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TLE4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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