TLR2 Antibody (T2.5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30097

Novus Biologicals, part of Bio-Techne
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NBP2-30097-50ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # T2.5

Concentration

0.5 mg/ml

View all TLR2 Antibodies »

Product Specifications

Immunogen

This antibody was raised against the extracellular domain of mouse TLR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TLR2 Antibody (T2.5)

Flow (Cell Surface): TLR2 Antibody (T2.5) [NBP2-30097]

Flow (Cell Surface): TLR2 Antibody (T2.5) [NBP2-30097]

Flow (Cell Surface): TLR2 Antibody (T2.5) [NBP2-30097] - Analysis using the FITC conjugate of NBP2-30097. Staining of TLR2 in 10^6 mouse bone marrow cells using 0.5 ug of NBP2-30096, 0.25 ug of anti-mouse CD11b, and 0.5 ug of isotype control.
Flow Cytometry: TLR2 Antibody (T2.5) [NBP2-30097]

Flow Cytometry: TLR2 Antibody (T2.5) [NBP2-30097]

Flow Cytometry: TLR2 Antibody (T2.5) [NBP2-30097] - Analysis using the FITC conjugate of NBP2-30097. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.

Applications for TLR2 Antibody (T2.5)

Application
Recommended Usage

Flow Cytometry

0.25 - 1.0ug 10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500
Application Notes
Use in Flow-cell surface reported in scientific literature (PMID 19091080)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (T2.5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TLR2 Antibody (T2.5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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