TMED9 Antibody (A9A7-R)

Western Blot: TMED9 Antibody (A9A7-R) [NBP3-33027]

Western Blot: TMED9 Antibody (A9A7-R) [NBP3-33027] - Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (NBP3-33027) at 1/1,000 dilution.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LO2 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: A431 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: Raji cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: Human lung tissue lysate
Lane 12: Mouse lung tissue lysate
Lane 13: Mouse liver tissue lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 10 seconds;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33027) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TMED9 Antibody (A9A7-R) [NBP3-33027]

Immunohistochemistry: TMED9 Antibody (A9A7-R) [NBP3-33027] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-TMED9 antibody (NBP3-33027) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33027) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.