TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16335

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16335-100ul
NBP3-16335-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6K5Q9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TMEFF2/Tomoregulin-2 Antibodies »

Product Summary for TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 275-374 of human TMEFF2/Tomoregulin-2 (Q9UIK5). HGKCEHSINMQEPSCRCDAGYTGQHCEKKDYSVLYVVPGPVRFQYVLIAAVIGTIQIAVICVVVLCITRKCPRSNRIHRQKQNTGHYSSDNTTRASTRLI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

Western Blot: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335]

Western Blot: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335]

Western Blot: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335] - Western blot analysis of extracts of various cell lines, using TMEFF2/Tomoregulin-2 Rabbit mAb (NBP3-16335) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335]

Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335]

Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody (6K5Q9) [NBP3-16335] - Immunohistochemistry of paraffin-embedded human placenta using TMEFF2/Tomoregulin-2 Rabbit mAb (NBP3-16335) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMEFF2/Tomoregulin-2

TMEFF2(transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor-like domain, had limited normal tissue distribution and was highly overexpressed in prostate cancer. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. Clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Alternate Names

TENB2, Tomoregulin-2, Tomoregulin2

Gene Symbol

TMEFF2

Additional TMEFF2/Tomoregulin-2 Products

Product Documents for TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TMEFF2/Tomoregulin-2 Antibody (6K5Q9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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