TMEM103 Antibody

Immunohistochemistry-Paraffin: TMEM103 Antibody [NBP1-91733]

Immunohistochemistry-Paraffin: C3orf75/ELP6 Antibody [NBP1-91733] - Staining of human pancreas shows distinct granular cytoplasmic positivity in exocrine glandular cells.

Western Blot: TMEM103 Antibody [NBP1-91733] -

Western Blot: TMEM103 Antibody [NBP1-91733] - The integrity & stability of the Elongator complex are required for gemcitabine-induced cytotoxic effects in GBC cells. a ELP5 depletion significantly downregulated the protein levels of other Elongator subunits, including ELP1, ELP2, ELP3, ELP4, ELP6. b ELP5 depletion significantly decreased ATPase activity in NOZ cells. c The abundance of thiolated tEUUC tRNA was decreased in ELP5-depleted NOZ cells. d Ectopically expressed ELP5 could interact with ELP3 & ELP4 in GBC cells. e Endogenous ELP3 & ELP4 degraded rapidly after treatment with 50 µg ml−1 cycloheximide (CHX) in ELP5-depleted GBC cells compared with WT cells. f–h Ectopically expressed ELP5 (f) could promote or rescue thiolated tRNA abundance (g) & GEM sensitivity (h) in WT & ELP5-depleted GBC cells, but ectopically expressed ELP3 or ELP4 (f) could only increase the abundance of thiolated tRNA (g) & GEM sensitivity (h) in WT cells, not in ELP5-depleted cells. i, j Mutation of the ATPase activity site residue within ELP5 (D124A) (i) could not promote GEM sensitivity in GBC cells (j). k, l Mutation of the cm5U catalytic activity site residue with ELP3 (C109/112 S) (k) could not promote GEM sensitivity in GBC cells (l). Data represent the mean ± S.D., n = 3 independent experiments in b, h, j, l, error bars represent S.D. Unpaired Student’s t-tests were used in b, h (NS, non-significant, **P < 0.01 & ***P < 0.001). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31792210), licensed under a CC-BY license. Not internally tested by Novus Biologicals.