TMEM143 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83809

Novus Biologicals, part of Bio-Techne
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NBP1-83809
NBP1-83809-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TMEM143 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MWNPREPRDWAQQYRERFIPFSKEQLLRLLIQEFHSSPAEKAALEAFSAHVDFCTLFHYHQILARLQALYDPINPDRETLDQPSLTDPQRLSNEQEVLRALEPLLAQANFSPLSEDTLAYALVVHHPQDEVQVTVNLDQYV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TMEM143 Antibody

Western Blot: TMEM143 Antibody [NBP1-83809]

Western Blot: TMEM143 Antibody [NBP1-83809]

Western Blot: TMEM143 Antibody [NBP1-83809] - Analysis in control (vector only transfected HEK293T lysate) and TMEM143 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TMEM143 Antibody [NBP1-83809]

Immunocytochemistry/ Immunofluorescence: TMEM143 Antibody [NBP1-83809]

Immunocytochemistry/Immunofluorescence: TMEM143 Antibody [NBP1-83809] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.
Immunohistochemistry-Paraffin: TMEM143 Antibody [NBP1-83809]

Immunohistochemistry-Paraffin: TMEM143 Antibody [NBP1-83809]

Immunohistochemistry-Paraffin: TMEM143 Antibody [NBP1-83809] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.

Applications for TMEM143 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TMEM143

Alternate Names

FLJ10922, transmembrane protein 143

Gene Symbol

TMEM143

Additional TMEM143 Products

Product Documents for TMEM143 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TMEM143 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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