TMPRSS4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-36997
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Chimpanzee (%), Monkey (%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
TMPRSS4 Antibody is made to a peptide with sequence HCFRKHTDVFNWK corresponding to internal region according to NP_063947.1, NP_001077416.1.
Reactivity Notes
Predicted cross-reactivity based on sequence identity: Chimpanzee (100%), Gibbon (100%), Marmoset (100%), Monkey (100%)
Specificity
TMPRSS4 Antibody is expected to recognise both reported isoforms (NP_063947.1 and NP_001077416.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for TMPRSS4 Antibody
![Western Blot: TMPRSS4 Antibody [NBP1-36997]](https://resources.bio-techne.com/images/products/TMPRSS4-Antibody-Western-Blot-NBP1-36997-img0002.jpg "Western Blot: TMPRSS4 Antibody [NBP1-36997]")
Western Blot: TMPRSS4 Antibody [NBP1-36997]
Western Blot: TMPRSS4 Antibody [NBP1-36997] - Analysis of TMPRSS4 in HEK293 lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. The observed molecular weight is represented by the band appearing at 48 kDa.Applications for TMPRSS4 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:1000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in lysates of HEK293 and A549 (calculated MW of 48.2 kDa band according to NP_063947.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TMPRSS4
TMPRSS4 has also been shown to play a role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 or potentially by TMPRSS4 at the cell surface to facilitate viral entry (3).
References
1. L de Aberasturi A, Calvo A. (2015) TMPRSS4: An Emerging Potential Therapeutic Target in Cancer. Br J Cancer. 112(1):4-8. PMID: 25203520
2. Zeng P., Zhang P., Zhou L., Tang M., Shen Y., Jin J., Zhu Y., Chen M. (2016) TMPRSS4 as an emerging potential poor prognostic factor for solid tumors: A systematic review and meta-analysis. Oncotarget. 7:76327-76336. PMID: 27344186
3. Zang F, Castro MFG, McCune BT, Zeng Q, Rothlauf PW, Sonnek NM, Liu Z, Brulois KF, Wang X, Greenberg HB, Diamond MS, Ciorba MA, Whelan SPJ, and Ding S. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes. Sci Immunol. 5(47): eabc3582. PMID: 32404436
Long Name
Transmembrane protease, serine 4
Alternate Names
CAPH2, MT-SP2
Entrez Gene IDs
56649 (Human)
Gene Symbol
TMPRSS4
UniProt
Additional TMPRSS4 Products
Product Documents for TMPRSS4 Antibody
Product Specific Notices for TMPRSS4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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