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TNIK Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13022

Novus Biologicals, part of Bio-Techne
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NBP3-13022

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TNIK Antibody

Western Blot: TNIK Antibody [NBP3-13022]

Western Blot: TNIK Antibody [NBP3-13022]

Western Blot: TNIK Antibody [NBP3-13022] - TNIK antibody validation by shRNA knock-down Upper panel: TNIK antibody (NBP3-13022) Lower panel: p84 antibody(NB100-174) A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of TNIK shRNA-transfected 293T cells 5% SDS-PAGE TNIK antibody (NBP3-13022) dilution: 1:5000 p84 antibody(NB100-174) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: TNIK Antibody [NBP3-13022]

Immunocytochemistry/ Immunofluorescence: TNIK Antibody [NBP3-13022]

Immunocytochemistry/Immunofluorescence: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TNIK protein stained by TNIK antibody (NBP3-13022) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]

Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022]

Immunohistochemistry-Paraffin: TNIK Antibody [NBP3-13022] - TNIK antibody detects TNIK protein at cytosol on Mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded Mouse small intestine TNIK antibody (NBP3-13022) dilution: 1:100 Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for TNIK Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS

Preservative

0.01% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TNIK

Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder, skin, and testis and cancerous bone, germ cell, nerve, and placenta.

Alternate Names

EC 2.7.11, KIAA0551EC 2.7.11.1, TRAF2 and NCK interacting kinase, TRAF2 and NCK-interacting protein kinase

Gene Symbol

TNIK

Additional TNIK Products

Product Documents for TNIK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TNIK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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