TNN Antibody (MTn-12)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08210
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # MTn-12
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Partially purified mouse tenascin.
Reactivity Notes
. No cross reactivity with tenascin of other species has been observed.
Localization
In immunohistological testing of frozen tissue sections of mouse intestine, the antibody stains the core of the villi but not the epithelial cells.
Specificity
This antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of approximate molecular weights 210 and 260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblasts lysates under reducing conditions.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for TNN Antibody (MTn-12)
![Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210]](https://resources.bio-techne.com/images/products/TNN-Antibody-MTn-12-Immunohistochemistry-Frozen-NBP3-08210-img0001.jpg "Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210]")
Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210]
Immunohistochemistry-Frozen: TNN Antibody (MTn-12) [NBP3-08210] - Frozen sections, acetone fixed of mouse intestine were stained with 1:400 monoclonal TNN Antibody (Clone: MTn-12) followed by 1:20 monoclonal Anti-Rat Kappa & Lambda Light Chains-FITC antibody produced in mouse and by 1:20 Anti-Mouse IgG (Fab specific)-FITC antibody produced in goat.Applications for TNN Antibody (MTn-12)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunohistochemistry
1:200
Immunohistochemistry-Frozen
1:200
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
IHC-Fr: In immunohistological testing of frozen tissue sections of mouse intestine, using immunofluorescent or immunoperoxidase staining, the antibody stains the core of the villi but not the epithelial cells. Dilution: 1:200.
ELISA: In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.
ELISA: In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.9% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TNN
Alternate Names
tenascin N, tenascin-N, tenascin-W, TN-N, TNW, TN-W
Gene Symbol
TNN
Additional TNN Products
Product Documents for TNN Antibody (MTn-12)
Product Specific Notices for TNN Antibody (MTn-12)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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