TNPO3 Antibody (0U3Q1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15908
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 0U3Q1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 824-923 of human Transportin 3 (TNPO3) (Q9Y5L0). QVMNQLGQQLVSQLLHTCCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGAVTVTHKQLTDFHKQVTSAEECKQVCWALRDFTRLFR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for TNPO3 Antibody (0U3Q1)
Western Blot: TNPO3 Antibody (0U3Q1) [NBP3-15908]
Western Blot: TNPO3 Antibody (0U3Q1) [NBP3-15908] - Western blot analysis of extracts of various cell lines, using Transportin 3 (TNPO3) Rabbit mAb (NBP3-15908) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunocytochemistry/ Immunofluorescence: TNPO3 Antibody (0U3Q1) [NBP3-15908]
Immunocytochemistry/Immunofluorescence: TNPO3 Antibody (0U3Q1) [NBP3-15908] - Immunofluorescence analysis of U-2 OS cells using Transportin 3 (TNPO3) Rabbit mAb (NBP3-15908) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: TNPO3 Antibody (0U3Q1) [NBP3-15908]
Immunohistochemistry-Paraffin: TNPO3 Antibody (0U3Q1) [NBP3-15908] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Transportin 3 (TNPO3) Rabbit mAb (NBP3-15908) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for TNPO3 Antibody (0U3Q1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TNPO3
Alternate Names
imp12, MTR10A, transportin 3, transportin-3
Gene Symbol
TNPO3
Additional TNPO3 Products
Product Documents for TNPO3 Antibody (0U3Q1)
Product Specific Notices for TNPO3 Antibody (0U3Q1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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