TOM1 Antibody (5A3)
Novus Biologicals, part of Bio-Techne | Catalog # H00010043-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5A3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TOM1 (NP_005479, 394 a.a. ~ 491 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GLAGALDARQQSTGAIPVTQACLMEDIEQWLSTDVGNDAEEPKGVTSEEFDKFLEERAKAADRLPNLSSPSAEGPPGPPSGPAPRKKTQEKDDDMLFA
Reactivity Notes
Human. Other species not tested.
Specificity
TOM1 (5A3)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for TOM1 Antibody (5A3)
![Western Blot: TOM1 Antibody (5A3) [H00010043-M01]](https://resources.bio-techne.com/images/products/TOM1-Antibody-5A3-Western-Blot-H00010043-M01-img0008.jpg "Western Blot: TOM1 Antibody (5A3) [H00010043-M01]")
Western Blot: TOM1 Antibody (5A3) [H00010043-M01]
Western Blot: TOM1 Antibody (5A3) [H00010043-M01] - TOM1 monoclonal antibody (M01), clone 5A3 Analysis of TOM1 expression in K-562.![Immunohistochemistry-Paraffin: TOM1 Antibody (5A3) [H00010043-M01]](https://resources.bio-techne.com/images/products/TOM1-Antibody-5A3-Immunohistochemistry-Paraffin-H00010043-M01-img0006.jpg "Immunohistochemistry-Paraffin: TOM1 Antibody (5A3) [H00010043-M01]")
Immunohistochemistry-Paraffin: TOM1 Antibody (5A3) [H00010043-M01]
Immunohistochemistry-Paraffin: TOM1 Antibody (5A3) [H00010043-M01] - Analysis of monoclonal antibody to TOM1 on formalin-fixed paraffin-embedded human skeletal muscle. Antibody concentration 3 ug/ml.![Western Blot: TOM1 Antibody (5A3) [H00010043-M01]](https://resources.bio-techne.com/images/products/TOM1-Antibody-5A3-Western-Blot-H00010043-M01-img0009.jpg "Western Blot: TOM1 Antibody (5A3) [H00010043-M01]")
Western Blot: TOM1 Antibody (5A3) [H00010043-M01]
Western Blot: TOM1 Antibody (5A3) [H00010043-M01] - Analysis of TOM1 expression in transfected 293T cell line by TOM1 monoclonal antibody (M01), clone 5A3.Lane 1: TOM1 transfected lysate(53.8 KDa).Lane 2: Non-transfected lysate.Applications for TOM1 Antibody (5A3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TOM1
Alternate Names
ADP-ribosylation factor-binding protein GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, golgi-associated, gamma adaptin ear containing, ARF binding protein 3, KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3
Entrez Gene IDs
10043 (Human)
Gene Symbol
TOM1
OMIM
604700 (Human)
UniProt
Additional TOM1 Products
Product Documents for TOM1 Antibody (5A3)
Product Specific Notices for TOM1 Antibody (5A3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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