TOM1 Antibody (PSH03-38)

Western Blot: TOM1 Antibody (PSH03-38) [NBP3-33035]

Western Blot: TOM1 Antibody (PSH03-38) [NBP3-33035] - Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/2,000 dilution.

Lane 1: 293T cell lysate (20 ug/Lane)
Lane 2: U-2 OS cell lysate (20 ug/Lane)
Lane 3: A431 cell lysate (20 ug/Lane)
Lane 4: K-562 cell lysate (20 ug/Lane)
Lane 5: HepG2 cell lysate (20 ug/Lane)
Lane 6: HeLa cell lysate (20 ug/Lane)
Lane 7: JAR cell lysate (20 ug/Lane)
Lane 8: THP-1 cell lysate (20 ug/Lane)
Lane 9: C2C12 cell lysate (20 ug/Lane)
Lane 10: PC-12 cell lysate (20 ug/Lane)
Lane 11: Mouse heart tissue lysate (40 ug/Lane)
Lane 12: Mouse skeletal muscle tissue lysate (40 ug/Lane)
Lane 13: Rat liver tissue lysate (40 ug/Lane)

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33035) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035]

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035] - Immunofluorescence analysis of paraffin-embedded human stomach carcinoma tissue labeling TOM1 with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33035, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035]

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.