Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TOM1 Antibodies »

Product Summary for TOM1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human target of myb1 using the numbering given in entry NP_005479.1 (GeneID 10043).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOM1 Antibody

Western Blot: TOM1 Antibody [NB100-61601]

Western Blot: TOM1 Antibody [NB100-61601]

Western Blot: TOM1 Antibody [NB100-61601] - Detection of Human TOM1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TOM1 antibody NB100-61601 used for IP at 3 mcg/mg lysate. TOM1 was also immunoprecipitated by rabbit anti- TOM1 antibody NB100-61602, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for TOM1 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TOM1

TOM1 (target of myb1) was originally identified as a gene activated by the expression of oncogenic Myb1. TOM1 has been shown to form a complex with tollip (toll-interacting protein) and to bind ubiquitin chains. It has also been shown to bind to clathrin heavy chain and is proposed to function to link polyubiquitinated proteins to clathrin and regulate endosomal trafficking of ubiquitinated proteins. Recent evidence shows that TOM1 bridges endofin to clathrin and is critical for the endosomal targeting of clathrin by endofin.

Alternate Names

ADP-ribosylation factor-binding protein GGA3, golgi associated, gamma adaptin ear containing, ARF binding protein 3, golgi-associated, gamma adaptin ear containing, ARF binding protein 3, KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Entrez Gene IDs

10043 (Human)

Gene Symbol

TOM1

UniProt

NP_005479.1 (Human)

Additional TOM1 Products

Product Documents for TOM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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