TOMM20 Antibody (10G6I8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15750

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15750-100ul
NBP3-15750-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10G6I8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TOMM20 Antibodies »

Product Summary for TOMM20 Antibody (10G6I8)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-145 of human TOMM20 (NP_055580.1). YCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TOMM20 Antibody (10G6I8)

Western Blot: TOMM20 Antibody (10G6I8) [NBP3-15750]

Western Blot: TOMM20 Antibody (10G6I8) [NBP3-15750]

Western Blot: TOMM20 Antibody (6E0N2) [NBP3-15750] - Western blot analysis of extracts of various cell lines, using TOMM20 antibody (NBP3-15750) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [NBP3-15750]

Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (10G6I8) [NBP3-15750]

Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (6E0N2) [NBP3-15750] - Confocal immunofluorescence analysis of HeLa cells using TOMM20 Rabbit mAb (NBP3-15750) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: TOMM20 Antibody (10G6I8) [NBP3-15750]

Immunohistochemistry-Paraffin: TOMM20 Antibody (10G6I8) [NBP3-15750]

Immunohistochemistry-Paraffin: TOMM20 Antibody (6E0N2) [NBP3-15750] - Immunohistochemistry of paraffin-embedded human colon carcinoma using TOMM20 antibody (NBP3-15750) at dilution of 1:400 - 3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for TOMM20 Antibody (10G6I8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Immunoprecipitation

-0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:2000 - 1:6000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TOMM20

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore

Long Name

TOMM20

Alternate Names

MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom

Gene Symbol

TOMM20

Additional TOMM20 Products

Product Documents for TOMM20 Antibody (10G6I8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TOMM20 Antibody (10G6I8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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