TOMM22 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80671
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQK
Reactivity Notes
Mouse reactivity reported in (PMID: 30386187). Rat (89%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for TOMM22 Antibody
Western Blot: TOMM22 Antibody [NBP1-80671]
Western Blot: TOMM22 Antibody [NBP1-80671] - Comparative immunoblot analysis of normal wild type versus mdx-4cv liver extracts. Immunoblots with expanded views of labelling with antibodies to the mitochondrial outer membrane protein translocation pore subunit TOM22 (NBP1-80671) and asporin (NBP2-15492). Image collected and cropped by CiteAb from the following publication (https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-018-9212-2) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: TOMM22 Antibody [NBP1-80671]
Immunocytochemistry/Immunofluorescence: TOMM22 Antibody [NBP1-80671] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining shown in green.Applications for TOMM22 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOMM22
Alternate Names
1C9-2, hTom22, mitochondrial import receptor subunit TOM22 homolog, mitochondrial import receptor Tom22, MST065, TOM22MSTP065, Translocase of outer membrane 22 kDa subunit homolog, translocase of outer mitochondrial membrane 22 homolog (yeast)
Entrez Gene IDs
56993 (Human)
Gene Symbol
TOMM22
UniProt
Additional TOMM22 Products
Product Documents for TOMM22 Antibody
Product Specific Notices for TOMM22 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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