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TOMM22 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80671

Novus Biologicals, part of Bio-Techne
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NBP1-80671
NBP1-80671-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAAAVAAAGAGEPQSPDELLPKGDAEKPEEELEEDDDEELDETLSERLWGLTEMFPERVRSAAGATFDLSLFVAQK

Reactivity Notes

Mouse reactivity reported in (PMID: 30386187). Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOMM22 Antibody

Western Blot: TOMM22 Antibody [NBP1-80671]

Western Blot: TOMM22 Antibody [NBP1-80671]

Western Blot: TOMM22 Antibody [NBP1-80671] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: TOMM22 Antibody [NBP1-80671]

Western Blot: TOMM22 Antibody [NBP1-80671]

Western Blot: TOMM22 Antibody [NBP1-80671] - Comparative immunoblot analysis of normal wild type versus mdx-4cv liver extracts. Immunoblots with expanded views of labelling with antibodies to the mitochondrial outer membrane protein translocation pore subunit TOM22 (NBP1-80671) and asporin (NBP2-15492). Image collected and cropped by CiteAb from the following publication (https://clinicalproteomicsjournal.biomedcentral.com/articles/10.1186/s12014-018-9212-2) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: TOMM22 Antibody [NBP1-80671]

Immunocytochemistry/ Immunofluorescence: TOMM22 Antibody [NBP1-80671]

Immunocytochemistry/Immunofluorescence: TOMM22 Antibody [NBP1-80671] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining shown in green.

Applications for TOMM22 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOMM22

TOMM22 is encoded by this gene is an integral membrane protein of the mitochondrial outer membrane. The encoded protein interacts with TOMM20 and TOMM40, and forms a complex with several other proteins to import cytosolic preproteins into the mitochondrion.

Alternate Names

1C9-2, hTom22, mitochondrial import receptor subunit TOM22 homolog, mitochondrial import receptor Tom22, MST065, TOM22MSTP065, Translocase of outer membrane 22 kDa subunit homolog, translocase of outer mitochondrial membrane 22 homolog (yeast)

Entrez Gene IDs

56993 (Human)

Gene Symbol

TOMM22

UniProt

Additional TOMM22 Products

Product Documents for TOMM22 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TOMM22 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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