TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47793

Novus Biologicals, part of Bio-Techne
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NBP2-47793-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # TOP1MT/488

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all TOP1MT Antibodies »

Product Summary for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Immunogen

Recombinant full-length human TOP1MT protein (Uniprot: Q969P6 )

Localization

Cytoplasmic (Mitochondria)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44360).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Western Blot: TOP1MT Antibody (TOP1MT/488)Azide and BSA Free [NBP2-47793]

Western Blot: TOP1MT Antibody (TOP1MT/488)Azide and BSA Free [NBP2-47793]

Western Blot: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of human Jurkat cell lysate using TOP1MT Antibody (TOP1MT/488).
Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793]

Immunohistochemistry-Paraffin: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793]

Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793]

Flow Cytometry: TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free [NBP2-47793] - Analysis of PFA-fixed MCF-7 cells using TOP1MT Antibody (TOP1MT/488).followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Applications for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TOP1MT

The TOP1MT gene encodes a mitochondrial DNA topoisomerase, an enzyme that controls and alters the topologic states of DNAduring transcription. This enzyme catalyzes the transient breaking and rejoining of DNA which allows the strands topass through one another

Alternate Names

mitochondrial, mitochondrial DNA topoisomerase I, topoisomerase (DNA) I, mitochondrial

Gene Symbol

TOP1MT

Additional TOP1MT Products

Product Documents for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TOP1MT Antibody (TOP1MT/488) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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