TOP2A Antibody (5B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26277

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26277-100ul
NBP3-26277-50ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TOP2A Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TOP2A [UniProt P11388]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOP2A Antibody (5B10)

TOP2A Antibody (5B10)

Immunohistochemistry: TOP2A Antibody (5B10) [NBP3-26277] -

Immunohistochemistry: TOP2A Antibody (5B10) [NBP3-26277] - Image of TOP2A Antibody (5B10) diluted at 1:100 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TOP2A Antibody (5B10)

Western Blot: TOP2A Antibody (5B10) [NBP3-26277] -

Western Blot: TOP2A Antibody (5B10) [NBP3-26277] - Positive Western Blot detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate.
All lanes: TOP2A Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 175, 178, 179, 183 kDa
Observed band size: 175 kDa

Applications for TOP2A Antibody (5B10)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

Additional TOP2A Products

Product Documents for TOP2A Antibody (5B10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TOP2A Antibody (5B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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