Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # TOP2A/1361

Concentration

0.2 mg/ml

View all TOP2A Antibodies »

Product Specifications

Immunogen

Recombinant human TOP2A fragment (aa1352-1493) (Uniprot: P11388)

Localization

Nuclear

Marker

Proliferation & Drug-Resistance Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54546)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TOP2A Antibody (TOP2A/1361)

Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Western Blot: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1361).
Immunocytochemistry/ Immunofluorescence: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Immunocytochemistry/ Immunofluorescence: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Immunocytochemistry/Immunofluorescence: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Confocal Immunofluorescence image of HeLa cells using TOP2A Antibody (TOP2A/1361) Green (CF488) and Phalloidin (Red) is used to label the nuclei.
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-53281]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1361) [NBP2-53281] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1361).
View 3 more images

Applications for TOP2A Antibody (TOP2A/1361)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

UniProt

P11388

Additional TOP2A Products

Product Documents for TOP2A Antibody (TOP2A/1361)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TOP2A Antibody (TOP2A/1361)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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