TOP2B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97789
![Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP2-97789]](https://resources.bio-techne.com/images/products/TOP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97789-img0001.jpg "Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP2-97789]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human TOP2B fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TOP2B Antibody
Immunocytochemistry/ Immunofluorescence: TOP2B Antibody [NBP2-97789]
Immunocytochemistry/Immunofluorescence: TOP2B Antibody [NBP2-97789] - Immunofluorescence staining of TOP2B in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TOP2B polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.Applications for TOP2B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TOP2B
Long Name
DNA Topoisomerase II beta
Alternate Names
antigen MLAA-44, DNA topoisomerase 2-beta, DNA topoisomerase II beta, DNA topoisomerase II, 180 kD, DNA topoisomerase II, beta isozyme, EC 5.99.1.3, top2beta, TOPIIB, topo II beta, topoisomerase (DNA) II beta (180kD), topoisomerase (DNA) II beta 180kDa, topoisomerase II beta, topoisomerase IIb, U937 associated antigen
Gene Symbol
TOP2B
Additional TOP2B Products
Product Specific Notices for TOP2B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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