Topo III beta Antibody (1H4)

Novus Biologicals, part of Bio-Techne | Catalog # H00008940-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1H4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Topo III beta Antibodies »

Product Specifications

Immunogen

TOP3B (AAH02432, 1 a.a. ~ 862 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKAHGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPYWANTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDMKKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARRPPGKPNPRRPKDKMSALAAYFV

Specificity

TOP3B - topoisomerase (DNA) III beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Topo III beta Antibody (1H4)

Western Blot: Topo III beta Antibody (1H4) [H00008940-M04]

Western Blot: Topo III beta Antibody (1H4) [H00008940-M04]

Western Blot: Topo III beta Antibody (1H4) [H00008940-M04] - Analysis of TOP3B expression in transfected 293T cell line by TOP3B monoclonal antibody (M04), clone 1H4. Lane 1: TOP3B transfected lysatE (96.662 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Topo III beta Antibody (1H4) [H00008940-M04]

Immunocytochemistry/ Immunofluorescence: Topo III beta Antibody (1H4) [H00008940-M04]

Immunocytochemistry/Immunofluorescence: Topo III beta Antibody (1H4) [H00008940-M04] - Analysis of monoclonal antibody to TOP3B on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Topo III beta Antibody (1H4) [H00008940-M04] - Detection limit for recombinant GST tagged TOP3B is 1 ng/ml as a capture antibody.

Applications for Topo III beta Antibody (1H4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Topo III beta

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described.

Alternate Names

DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, EC 5.99.1.2, FLJ39376, TOP3B1, topoisomerase (DNA) III beta, topoisomerase III beta

Entrez Gene IDs

8940 (Human)

Gene Symbol

TOP3B

OMIM

603582 (Human)

UniProt

AAH02432 (Human)

Additional Topo III beta Products

Product Documents for Topo III beta Antibody (1H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Topo III beta Antibody (1H4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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