TOPORS Antibody (5G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00010210-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TOPORS Antibodies »

Product Specifications

Immunogen

TOPORS (NP_005793, 98 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPDSKCPICLDRFDNVSYLDRCLHKFCFRCVQEWSKNKAECPLCKQPFDSIFHSVRAEDDFKEYVLRPSYNGSFVTPDRRFRYRTTLTRERNASVYSPSGPVNRRTTT

Reactivity Notes

Other species not tested.

Specificity

TOPORS - topoisomerase I binding, arginine/serine-rich

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TOPORS Antibody (5G11)

Western Blot: TOPORS Antibody (5G11) [H00010210-M01]

Western Blot: TOPORS Antibody (5G11) [H00010210-M01]

Western Blot: TOPORS Antibody (5G11) [H00010210-M01] - TOPORS expression in HeLa NE ( Cat # L013V3 ).
Immunocytochemistry/ Immunofluorescence: TOPORS Antibody (5G11) [H00010210-M01]

Immunocytochemistry/ Immunofluorescence: TOPORS Antibody (5G11) [H00010210-M01]

Immunocytochemistry/Immunofluorescence: TOPORS Antibody (5G11) [H00010210-M01] - Analysis of monoclonal antibody to TOPORS on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TOPORS Antibody (5G11) [H00010210-M01]

Immunohistochemistry-Paraffin: TOPORS Antibody (5G11) [H00010210-M01]

Immunohistochemistry-Paraffin: TOPORS Antibody (5G11) [H00010210-M01] - Analysis of monoclonal antibody to TOPORS on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.
View 1 more images

Applications for TOPORS Antibody (5G11)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TOPORS

TOPORS functions as an ubiquitin-protein E3 ligase and as an E3 SUMO1-protein ligase. Can both ubiquitinate and sumoylate p53

Alternate Names

EC 6.3.2.-, LUNtopoisomerase I binding, arginine/serine-rich, p53-binding protein 3, P53BP3, retinitis pigmentosa 31 (autosomal dominant), RP31, SUMO1-protein E3 ligase Topors, topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase, Topoisomerase I-binding arginine/serine-rich protein, Topoisomerase I-binding RING finger protein, TP53BPLE3 ubiquitin-protein ligase Topors, Tumor suppressor p53-binding protein 3

Entrez Gene IDs

10210 (Human)

Gene Symbol

TOPORS

UniProt

NP_005793 (Human)

Additional TOPORS Products

Product Documents for TOPORS Antibody (5G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TOPORS Antibody (5G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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