TORC3/CRTC3 Antibody (JE63-18)

Western Blot: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226]

Western Blot: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Western blot analysis of TORC3/CRTC3 on different lysates with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: TT cell lysate
Lane 3: Jurkat cell lysate
Lane 4: HDLM-2 cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: RAW264.7 cell lysate
Lane 8: NIH/3T3 cell lysate
Lane 9: PC-12 cell lysate
Lane 10: C6 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 67 kDa
Observed band size: 75 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32226) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226]

Immunohistochemistry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-TORC3/CRTC3 antibody (NBP3-32226) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32226) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226]

Flow Cytometry: TORC3/CRTC3 Antibody (JE63-18) [NBP3-32226] - Flow cytometric analysis of TT cells labeling TORC3/CRTC3.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32226, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).