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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 156 to 206 of human Tumor Protein D52-like 2 using the numbering given in entry NP_003279.2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPD52L2/D54 Antibody

Western Blot: TPD52L2/D54 Antibody [NBP2-36548]

Western Blot: TPD52L2/D54 Antibody [NBP2-36548]

Western Blot: TPD52L2/D54 Antibody [NBP2-36548] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TPD52L2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: TPD52L2/D54 Antibody [NBP2-36548]

Immunoprecipitation: TPD52L2/D54 Antibody [NBP2-36548]

Immunoprecipitation: TPD52L2/D54 Antibody [NBP2-36548] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated TPD52L2, it was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TPD52L2/D54 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TPD52L2/D54

Alternate Names

DKFZp686A1765, HCCR-binding protein 2, tumor protein D52-like 2hD54D54, tumor protein D54

Gene Symbol

TPD52L2

Additional TPD52L2/D54 Products

Product Documents for TPD52L2/D54 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TPD52L2/D54 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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