TPK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83152

Novus Biologicals, part of Bio-Techne
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NBP1-83152
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TPK1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPK1 Antibody

Western Blot: TPK1 Antibody [NBP1-83152]

Western Blot: TPK1 Antibody [NBP1-83152]

Western Blot: TPK1 Antibody [NBP1-83152] - Analysis in control (vector only transfected HEK293T lysate) and TPK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TPK1 Antibody [NBP1-83152]

Immunocytochemistry/ Immunofluorescence: TPK1 Antibody [NBP1-83152]

Immunocytochemistry/Immunofluorescence: TPK1 Antibody [NBP1-83152] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83152]

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83152]

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83152] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
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Applications for TPK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPK1

TPK1 encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

EC 2.7.6.2, HTPK1, Placental protein 20, PP20thiamine kinase, thiamin pyrophosphokinase 1, thiamine diphosphokinase, Thiamine pyrophosphokinase 1

Gene Symbol

TPK1

Additional TPK1 Products

Product Documents for TPK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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