TPMT Antibody (1D4)
Novus Biologicals, part of Bio-Techne | Catalog # H00007172-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1D4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
TPMT (AAH05339, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRRLEKVDAFEERHKSWGIDCLFEKLYLLTEK
Specificity
TPMT - thiopurine S-methyltransferase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for TPMT Antibody (1D4)
![Western Blot: TPMT Antibody (1D4) [H00007172-M02]](https://resources.bio-techne.com/images/products/TPMT-Antibody-1D4-Western-Blot-H00007172-M02-img0007.jpg "Western Blot: TPMT Antibody (1D4) [H00007172-M02]")
Western Blot: TPMT Antibody (1D4) [H00007172-M02]
Western Blot: TPMT Antibody (1D4) [H00007172-M02] - Analysis of TPMT expression in transfected 293T cell line by TPMT monoclonal antibody (M02), clone 1D4. Lane 1: TPMT transfected lysate(28.2 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02]](https://resources.bio-techne.com/images/products/TPMT-Antibody-1D4-Immunocytochemistry-Immunofluorescence-H00007172-M02-img0005.jpg "Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02]")
Immunocytochemistry/ Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02]
Immunocytochemistry/Immunofluorescence: TPMT Antibody (1D4) [H00007172-M02] - Analysis of monoclonal antibody to TPMT on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: TPMT Antibody (1D4) [H00007172-M02] - Detection limit for recombinant GST tagged TPMT is approximately 1ng/ml as a capture antibody.
Applications for TPMT Antibody (1D4)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IF and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: TPMT
Long Name
Thiopurine S-methyltransferase
Alternate Names
EC 2.1.1.67
Entrez Gene IDs
7172 (Human)
Gene Symbol
TPMT
OMIM
187680 (Human)
UniProt
Additional TPMT Products
Product Documents for TPMT Antibody (1D4)
Product Specific Notices for TPMT Antibody (1D4)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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