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TPPP/p25 Antibody (3S5U9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16454

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16454-100ul
NBP3-16454-20ul

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3S5U9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TPPP/p25 (O94811). FSKIKGKSCRTITFEQFQEALEELAKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHKERFDPSGKGKGKAGRVDLVDESG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPPP/p25 Antibody (3S5U9)

Western Blot: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Western Blot: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Western Blot: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Western blot analysis of extracts of various cell lines, using TPPP/p25 Rabbit mAb (NBP3-16454) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Immunohistochemistry of paraffin-embedded mouse brain using TPPP/p25 Rabbit mAb (NBP3-16454) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody (3S5U9) [NBP3-16454] - Immunohistochemistry of paraffin-embedded rat brain using TPPP/p25 Rabbit mAb (NBP3-16454) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Applications for TPPP/p25 Antibody (3S5U9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TPPP/p25

TPPP may play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity ofthe microtubule network. May play a role in mitotic spindle assembly and nuclear env

Alternate Names

p24, P24,25 kDa brain-specific protein, P25, p25alpha, p25-alpha, TPPP/p25brain specific protein p25 alpha, TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization promoting protein, tubulin polymerization-promoting protein

Gene Symbol

TPPP

Additional TPPP/p25 Products

Product Documents for TPPP/p25 Antibody (3S5U9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TPPP/p25 Antibody (3S5U9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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