TPPP/p25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49833

Novus Biologicals, part of Bio-Techne
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NBP1-49833
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all TPPP/p25 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DPSKDRAAKRLSLES corresponding to internal region according to NP_008961.1.

Epitope

DPSKDRAAKRLSLES - Internal region

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TPPP/p25 Antibody

Western Blot: TPPP/p25 Antibody [NBP1-49833]

Western Blot: TPPP/p25 Antibody [NBP1-49833]

Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) staining of Mouse (A) and Rat (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP1-49833] - Staining of Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: TPPP/p25 Antibody [NBP1-49833]

Western Blot: TPPP/p25 Antibody [NBP1-49833]

Western Blot: TPPP/p25 Antibody [NBP1-49833] - (0.001ug/ml) Analysis of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Applications for TPPP/p25 Antibody

Application
Recommended Usage

Immunohistochemistry

3.75 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.0001-0.01 ug/ml
Application Notes
WB: Approx. 26 kDa band observed in human brain is reported in scientific literature (PMID: 18028908). An additional band of unknown identity was also consistently observed at 17 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human cerebral cortex shows cytoskeletal-like staining throughout the fibre network.

Reviewed Applications

Read 1 review rated 4 using NBP1-49833 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TPPP/p25

TPPP may play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity ofthe microtubule network. May play a role in mitotic spindle assembly and nuclear env

Alternate Names

p24, P24,25 kDa brain-specific protein, P25, p25alpha, p25-alpha, TPPP/p25brain specific protein p25 alpha, TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization promoting protein, tubulin polymerization-promoting protein

Entrez Gene IDs

11076 (Human); 72948 (Mouse); 361466 (Rat)

Gene Symbol

TPPP

UniProt

NP_008961.1 (Human)

Additional TPPP/p25 Products

Product Documents for TPPP/p25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPPP/p25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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