TPPP/p25 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34031

Novus Biologicals, part of Bio-Techne
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NBP2-34031
NBP2-34031-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TPPP/p25 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKFTGSHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPPP/p25 Antibody

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Analysis in human cerebral cortex and liver tissues using NBP2-34031 antibody. Corresponding TPPP RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031]

Immunohistochemistry-Paraffin: TPPP/p25 Antibody [NBP2-34031] - Staining of human cerebral cortex, liver, lower gastrointestinal and pancreas using Anti-TPPP antibody NBP2-34031 (A) shows similar protein distribution across tissues to independent antibody NBP1-80962 (B).
Western Blot: TPPP/p25 Antibody [NBP2-34031]

Western Blot: TPPP/p25 Antibody [NBP2-34031]

Western Blot: TPPP/p25 Antibody [NBP2-34031] - TPPP/p25, a microtubule-stabilising protein, is a target of linc00899 in regulation of mitosis. Representative western blot of TPPP/p25 levels after depletion of linc00899. beta-Tubulin and p150 were used as two loading controls. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-020-14978-7), licensed under a CC-BY license.
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Applications for TPPP/p25 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 5 using NBP2-34031 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPPP/p25

TPPP may play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity ofthe microtubule network. May play a role in mitotic spindle assembly and nuclear env

Alternate Names

p24, P24,25 kDa brain-specific protein, P25, p25alpha, p25-alpha, TPPP/p25brain specific protein p25 alpha, TPPP1glycogen synthase kinase 3 (GSK3) inhibitor p24, tubulin polymerization promoting protein, tubulin polymerization-promoting protein

Gene Symbol

TPPP

UniProt

O94811 (Human)

Additional TPPP/p25 Products

Product Documents for TPPP/p25 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPPP/p25 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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