TPT1/TCTP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25204

Novus Biologicals, part of Bio-Techne
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NBP3-25204
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TPT1/TCTP Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein TPT1/TCTP using the following amino acid sequence: AAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKCVSTRKWV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPT1/TCTP Antibody

TPT1/TCTP Antibody Immunohistochemistry-Paraffin: TPT1/TCTP Antibody [NBP3-25204]

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody [NBP3-25204]

Staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells.
TPT1/TCTP Antibody Western Blot: TPT1/TCTP Antibody [NBP3-25204]

Western Blot: TPT1/TCTP Antibody [NBP3-25204]

Analysis in human cell line RT-4 and human cell line U-251 MG.

Applications for TPT1/TCTP Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TPT1/TCTP

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Gene Symbol

TPT1

Additional TPT1/TCTP Products

Product Documents for TPT1/TCTP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPT1/TCTP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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