TPX2 Antibody (TPX2-01) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67265

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TPX2-01

Format

BSA Free

Concentration

1 mg/ml

View all TPX2 Antibodies »

Product Specifications

Immunogen

Human TPX2

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for TPX2 Antibody (TPX2-01) - BSA Free

Western Blot: TPX2 Antibody (TPX2-01)BSA Free [NBP2-67265]

Western Blot: TPX2 Antibody (TPX2-01)BSA Free [NBP2-67265]

Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.
Immunocytochemistry: TPX2 Antibody (TPX2-01) - BSA Free [NBP2-67265]

Immunocytochemistry: TPX2 Antibody (TPX2-01) - BSA Free [NBP2-67265]

Immunocytochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - Glioblastoma cell line T98G: a) interphase, b) mitosis. Bar: 20 um.
Immunohistochemistry: TPX2 Antibody (TPX2-01) - BSA Free [NBP2-67265]

Immunohistochemistry: TPX2 Antibody (TPX2-01) - BSA Free [NBP2-67265]

Immunohistochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.
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Applications for TPX2 Antibody (TPX2-01) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TPX2

TPX2 is a microtubule cross-linking protein involved in spindle formation. During mitosis, it is strictly associated with the mitotic spindle, whereas during phases S and G2 it is diffusely distributed throughout the nucleus.

Alternate Names

C20orf1HCTP4, C20orf2, chromosome 20 open reading frame 1, Differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2FLS353, HCA519, Hepatocellular carcinoma-associated antigen 519, P100, p100GD:C20orf1, preferentially expressed in colorectal cancer, Protein fls353, REPP86, restricted expression proliferation associated protein 100, Restricted expression proliferation-associated protein 100, targeting protein for Xklp2, TPX2, microtubule-associated protein homolog, TPX2, microtubule-associated, homolog (Xenopus laevis)

Gene Symbol

TPX2

Additional TPX2 Products

Product Documents for TPX2 Antibody (TPX2-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TPX2 Antibody (TPX2-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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