Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TPX2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 150 and 200 of human TPX2 using the numbering given in Swiss-Prot entry Q9ULW0 (GeneID 22974).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TPX2 Antibody

Western Blot: TPX2 Antibody [NB500-183]

Western Blot: TPX2 Antibody [NB500-183]

Western Blot: TPX2 Antibody [NB500-183] - Detection of Human TPX2 on HeLa whole cell lysate using NB500-183. TPX2 was also immunoprecipitated using NB500-184 at 0.4 ug/mg lysate.
Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-183]

Immunocytochemistry/ Immunofluorescence: TPX2 Antibody [NB500-183]

Immunocytochemistry/Immunofluorescence: TPX2 Antibody [NB500-183] - HeLa cell. Antibody: Affinity purified rabbit anti- TPX2 used at a dilution of 1:400 (2.5ug/ml). Detection: Red-fluorescent goat anti-rabbit IgGheavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Applications for TPX2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250 to 1:1000

Immunoprecipitation

1-4 ug/mg lysate

Western Blot

1:5000-1:15000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TPX2

DAZL is a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localised to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males

Alternate Names

C20orf1HCTP4, C20orf2, chromosome 20 open reading frame 1, Differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in lung cells, DIL2, DIL-2FLS353, HCA519, Hepatocellular carcinoma-associated antigen 519, P100, p100GD:C20orf1, preferentially expressed in colorectal cancer, Protein fls353, REPP86, restricted expression proliferation associated protein 100, Restricted expression proliferation-associated protein 100, targeting protein for Xklp2, TPX2, microtubule-associated protein homolog, TPX2, microtubule-associated, homolog (Xenopus laevis)

Entrez Gene IDs

22974 (Human)

Gene Symbol

TPX2

UniProt

Q9ULW0 (Human)

Additional TPX2 Products

Product Documents for TPX2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TPX2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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