Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TR2/NR2C1 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 1 and 50 of human Testicular Receptor 2 using the numbering given in entry NP_003288.2 (GeneID 7181).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TR2/NR2C1 Antibody

Western Blot: TR2/NR2C1 Antibody [NBP1-71803]

Western Blot: TR2/NR2C1 Antibody [NBP1-71803]

Western Blot: TR2/NR2C1 Antibody [NBP1-71803] - Whole cell lysate from HeLa (H; 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (5, 15, and 50 mcg for WB) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-TR2 antibody used for WB at 0.1 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TR2 was also immunoprecipitated by rabbit anti-TR2 antibody NBP1-71804, which recognizes a downstream epitope.

Applications for TR2/NR2C1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 mcg/mg

Western Blot

1:2000-1:10000
Application Notes
Use in Immunohistochemistry reported in scientific literature (PMID 28551284).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TR2/NR2C1

This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Long Name

Testicular Orphan Nuclear Receptor 2

Alternate Names

NR2C1

Gene Symbol

NR2C1

UniProt

NP_003288.2 (Human)

Additional TR2/NR2C1 Products

Product Documents for TR2/NR2C1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TR2/NR2C1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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