TR4/NR2C2 Antibody (2A5)

Novus Biologicals, part of Bio-Techne | Catalog # H00007182-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TR4/NR2C2 Antibodies »

Product Specifications

Immunogen

NR2C2 (NP_003289, 43 a.a. ~ 152 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KIVTDQQTGQKIQIVTAVDASGSPKQQFILTSPDGAGTGKVILASPETSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKTDVQRPQVVEYCVVCGDKASGRHYGAVS

Localization

Nuclear

Specificity

NR2C2 - nuclear receptor subfamily 2, group C, member 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TR4/NR2C2 Antibody (2A5)

Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of NR2C2 over-expressed 293 cell line, cotransfected with NR2C2 Validated Chimera RNAi ( Cat # H00007182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NR2C2 monoclonal antibody (M01), clone 2A5 (Cat # H00007182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of monoclonal antibody to NR2C2 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.
Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01]

Western Blot: TR4/NR2C2 Antibody (2A5) [H00007182-M01] - Analysis of NR2C2 expression in transfected 293T cell line by NR2C2 monoclonal antibody (M01), clone 2A5.Lane 1: NR2C2 transfected lysate(65.414 KDa).Lane 2: Non-transfected lysate.

Applications for TR4/NR2C2 Antibody (2A5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TR4/NR2C2

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Long Name

Testicular Orphan Nuclear Receptor 4

Alternate Names

NR2C2, TAK1

Entrez Gene IDs

7182 (Human)

Gene Symbol

NR2C2

OMIM

601426 (Human)

UniProt

NP_003289 (Human)

Additional TR4/NR2C2 Products

Product Documents for TR4/NR2C2 Antibody (2A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TR4/NR2C2 Antibody (2A5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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