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TR4/NR2C2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97138

Novus Biologicals, part of Bio-Techne
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NBP2-97138-100ul
NBP2-97138-50ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human TR4/NR2C2 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for TR4/NR2C2 Antibody

Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP2-97138]

Immunocytochemistry/ Immunofluorescence: TR4/NR2C2 Antibody [NBP2-97138]

Immunocytochemistry/Immunofluorescence: TR4/NR2C2 Antibody [NBP2-97138] - Immunofluorescence staining of TR4/NR2C2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human TR4/NR2C2 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138] - Immunochemical staining of human TR4/NR2C2 in human skin with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138]

Immunohistochemistry-Paraffin: TR4/NR2C2 Antibody [NBP2-97138] - Immunochemical staining of human TR4/NR2C2 in human lymph node with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Applications for TR4/NR2C2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TR4/NR2C2

TR4 (TAK1, NR2C2) is an orphan nuclear receptor. TR4 was originally cloned from lymphoblastoma Raji cells or mouse brain cDNA library. No ligand has been reported. Northern blot shows TR4 is transcribed as a 9kb mRNA in many tissues and as a 2.8kb mRNA in testis, mainly in spermatocytes. TR4 has two isoforms called TR4alpha1 and TR4 alpha2,which differ in 19 amino acids coded by two separate exons. Both products translated from the 9kb transcript are ubiquitously expressed. Since TR4 binds to the same elements for the RAR-RXR or TR-RXR heterodimers, TR4 may have an inhibitory effect for retinoic-acid mediated transactivation.

Long Name

Testicular Orphan Nuclear Receptor 4

Alternate Names

NR2C2, TAK1

Gene Symbol

NR2C2

Additional TR4/NR2C2 Products

Product Documents for TR4/NR2C2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TR4/NR2C2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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