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TRAF-4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56177

Novus Biologicals, part of Bio-Techne
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NB100-56177
NB100-56177SS

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Zebrafish

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 1-17 (MPGFDYKFLEKPKRRLL) of human TRAF4 was used as immunogen; GenBank no. gi|22027622|ref|NP_004286.2|.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-4 Antibody

Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177]

Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177]

Immunohistochemistry-Paraffin: TRAF-4 Antibody [NB100-56177] - Formalin-fixed, paraffin embedded, normal human mammary epithelial tissue using th antibody. A-C: Strong TRAF4 staining is seen in the luminal epithelial cells of the interlobular terminal ducts (acini). However, there is absence of TRAF4 immunostaining in the myoepithelial cells along the basement membrane. B and C: higher magnifications of A. Data courtesy of Krajewska et al.
Immunoprecipitation: TRAF-4 Antibody [NB100-56177]

Immunoprecipitation: TRAF-4 Antibody [NB100-56177]

Immunoprecipitation: TRAF-4 Antibody [NB100-56177] - The NB100-56177 TRAF4 antibody specifically immunoprecipitates TRAF4. Top: TRAFs 2,3,4,5, and 6 were in vitro translated in the presence of 35S-methionine, and immunoprecipiated using the TRAF4 antibody. The TRAF4 antibody immunoprecipitated only TRAF4 and did not recognize TRAFs 2, 3, 5, or 6. A reticulocyte primed with an empty pcDNA plasmid was used as a negative control. Bottom: SDS-PAGE visualization of the in vitro translated TRAFs. Data courtesy of Krajewska et al.

Applications for TRAF-4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:1000-1:5000

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-4

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis.

Long Name

TNF Receptor-Associated Factor 4

Alternate Names

CART1, MLN62, TRAF4

Entrez Gene IDs

9618 (Human); 22032 (Mouse); 303285 (Rat)

Gene Symbol

TRAF4

Additional TRAF-4 Products

Product Documents for TRAF-4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRAF-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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