TRAF3IP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56740

Novus Biologicals, part of Bio-Techne
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NB100-56740
NB100-56740SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRAF3IP2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a synthetic peptide (QDLPRPLRSREFPQFEP) corresponding to amino acids 225-241 of human TRAF3IP2.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for these species: human (100%), rat (88%), and mouse (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF3IP2 Antibody

Western Blot: TRAF3IP2 Antibody [NB100-56740]

Western Blot: TRAF3IP2 Antibody [NB100-56740]

Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of CIKS using NB100-56740 on 15 ugs of mouse kidney cell lysate.
Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740]

Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740]

Immunohistochemistry-Paraffin: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney tissue using ACT1 antibody at 5 ug/ml.
Western Blot: TRAF3IP2 Antibody [NB100-56740]

Western Blot: TRAF3IP2 Antibody [NB100-56740]

Western Blot: TRAF3IP2 Antibody [NB100-56740] - Analysis of ACT1 in human kidney lysate in the A) absence and B) presence of immunizing peptide using ACT1 antibody at 2 ug/ml.
View 1 more images

Applications for TRAF3IP2 Antibody

Application
Recommended Usage

Immunoprecipitation

reported in scientific literature (PMID 24561578)

Simple Western

1:20

Western Blot

1-2 ug/ml
Application Notes
An approx. 60 kDa band is observed. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF3IP2

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (3) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1/CIKS cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1/CIKS protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

Alternate Names

ACT1DKFZP586G0522, adapter protein CIKS, C6orf2, C6orf4chromosome 6 open reading frame 5, C6orf5DKFZp586G0522, C6orf6MGC3581, CIKSchromosome 6 open reading frame 2, Connection to IKK and SAPK/JNK, NFkB-activating protein ACT1, Nuclear factor NF-kappa-B activator 1, TRAF3 interacting protein 2, TRAF3-interacting protein 2

Entrez Gene IDs

103213 (Mouse); 10758 (Rat); 361857 (Rat)

Gene Symbol

TRAF3IP2

UniProt

NP_679211 (Human)

Additional TRAF3IP2 Products

Product Documents for TRAF3IP2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for TRAF3IP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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