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TRAF7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-1399

Novus Biologicals, part of Bio-Techne
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NB600-1399SS
NB600-1399

Key Product Details

Species Reactivity

Human, Mouse, Rat, Equine

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Amino acids 334-356 of human TRAF7 protein were used as the immunogen for this antibody.

Epitope

ELKFDVLDENQSKLSEDLMEFRR

Localization

Colocalizes with MAP3K3 to vesicle like structures throughout the cytoplasm.

Specificity

Human (isoforms CRA_a and CRA_c).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF7 Antibody

Western Blot: TRAF7 Antibody [NB600-1399]

Western Blot: TRAF7 Antibody [NB600-1399]

Western Blot: TRAF7 Antibody [NB600-1399] - Total protein from Huvec, MCF7, U2-OS, A431, SHSY-5Y and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-TRAF7 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Applications for TRAF7 Antibody

Application
Recommended Usage

Immunoprecipitation

reported in scientific literature (Zotti et al (2011))

Western Blot

2 ug/ml. Use reported in scientific literature (Zotti et al (2011))

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF7

Tumor necrosis factor (TNF; see MIM 191160) receptor-associated factors, such as TRAF7, are signal transducers for members of the TNF receptor superfamily (see MIM 191190). TRAFs are composed of an N-terminal cysteine/histidine-rich region containing zinc RING and/or zinc finger motifs; a coiled-coil (leucine zipper) motif; and a homologous region that defines the TRAF family, the TRAF domain, which is involved in self-association and receptor binding

Alternate Names

DKFZp586I021, E3 ubiquitin-protein ligase TRAF7, EC 6.3.2.-, MGC7807, RFWD1RING finger protein 119, RNF119RING finger and WD repeat-containing protein 1, TNF receptor-associated factor 7ring finger and WD repeat domain 1

Entrez Gene IDs

84231 (Human); 360491 (Rat)

Gene Symbol

TRAF7

Additional TRAF7 Products

Product Documents for TRAF7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRAF7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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