Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8C8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Transgelin/TAGLN Antibodies »

Product Specifications

Immunogen

Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8)Azide and BSA Free [NBP2-74584]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8)Azide and BSA Free [NBP2-74584]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SM22 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SM22 alpha.
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584]

Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584]

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584]

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584]

Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Human prostate tissue using anti-SM22 alpha mouse monoclonal antibody.
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Applications for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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