Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20101
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-33B-129
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Peptide "AAEDYGVIK", derived from the actin cross-linking protein Transgelin, conjugated to KLH.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -
Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-33B-129 (NBP3-20101) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20101, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - HeLa(A), Caco-2(B), U2OS(C) cell lysate and human placenta(D), muscle(E) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-33B-129 (NBP3-20101) at 0.0003 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] -
Flow Cytometry: Transgelin/TAGLN/SM22 alpha Antibody (SAIC-33B-129) - Azide and BSA Free [NBP3-20101] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-33B-129 (NBP3-20101, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.Applications
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Mass Spectrometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Transgelin/TAGLN
Alternate Names
SM22 alpha, TAGLN, WS3-10
Gene Symbol
TAGLN
Additional Transgelin/TAGLN Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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