Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47688
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Porcine, Bovine, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # TAGLN/247
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Transgelin/TAGLN/SM22 alpha protein. (Uniprot: Q01995)
Localization
Cytoplasmic
Specificity
This monoclonal antibody recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44435).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688]
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688]
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free [NBP2-47688] - Human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247)
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Transgelin/TAGLN/SM22 alpha antibody (TAGLN/247).Applications for Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Transgelin/TAGLN
Alternate Names
SM22 alpha, TAGLN, WS3-10
Gene Symbol
TAGLN
Additional Transgelin/TAGLN Products
Product Documents for Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free
Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody (TAGLN/247) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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