Transgelin/TAGLN/SM22 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33003

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (97%), Porcine (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Transgelin/TAGLN Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transgelin/TAGLN/SM22 alpha. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (85%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody

Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Immunocytochemistry/Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin protein stained by Transgelin antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - Mouse smooth muscle. Transgelin stained by Transgelin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP1-33003] - 30 ug C8D30 whole cell lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.
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Applications for Transgelin/TAGLN/SM22 alpha Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Alternate Names

SM22 alpha, TAGLN, WS3-10

Entrez Gene IDs

6876 (Human)

Gene Symbol

TAGLN

UniProt

Q01995

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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