Transgelin/TAGLN/SM22 alpha Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25205

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-25205
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Transgelin/TAGLN Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Transgelin/TAGLN/SM22 alpha using the following amino acid sequence: KNDGHYRGDPNWFMKKAQEHKREFTESQLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transgelin/TAGLN/SM22 alpha Antibody

Transgelin/TAGLN/SM22 alpha Antibody Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP3-25205]

Immunohistochemistry-Paraffin: Transgelin/TAGLN/SM22 alpha Antibody [NBP3-25205]

Staining of human breast shows cytoplasmic positivity in myoepithelial cells.
Transgelin/TAGLN/SM22 alpha Antibody Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP3-25205]

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody [NBP3-25205]

Analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.

Applications for Transgelin/TAGLN/SM22 alpha Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 µg/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transgelin/TAGLN

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Alternate Names

SM22 alpha, TAGLN, WS3-10

Gene Symbol

TAGLN

Additional Transgelin/TAGLN Products

Product Documents for Transgelin/TAGLN/SM22 alpha Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Transgelin/TAGLN/SM22 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.