Transketolase Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12646
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Transketolase Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human Transketolase fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for Transketolase Antibody
![Western Blot: Transketolase Antibody [NBP3-12646]](https://resources.bio-techne.com/images/products/Transketolase-Antibody-Western-Blot-NBP3-12646-img0001.jpg "Western Blot: Transketolase Antibody [NBP3-12646]")
Western Blot: Transketolase Antibody [NBP3-12646]
Western Blot: Transketolase Antibody [NBP3-12646] - Lane A: HepG2 Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:68 kDaObserved band size:70 kDa![Immunoprecipitation: Transketolase Antibody [NBP3-12646]](https://resources.bio-techne.com/images/products/Transketolase-Antibody-Immunoprecipitation-NBP3-12646-img0002.jpg "Immunoprecipitation: Transketolase Antibody [NBP3-12646]")
Immunoprecipitation: Transketolase Antibody [NBP3-12646]
Immunoprecipitation: Transketolase Antibody [NBP3-12646] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-TKT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TKT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size :68 kDaApplications for Transketolase Antibody
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Transketolase
Alternate Names
EC 2.2.1.1, FLJ34765, TK, TKT1, transketolase
Gene Symbol
TKT
Additional Transketolase Products
Product Specific Notices for Transketolase Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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