Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3H4-2H6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRAP1 Antibodies »

Product Specifications

Immunogen

Purified recombinant TRAP1

Localization

Mitochondrion

Specificity

Detects 75kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRAP1 Antibody (3H4-2H6)

Western Blot: TRAP1 Antibody (3H4-2H6) [NBP2-59343]

Western Blot: TRAP1 Antibody (3H4-2H6) [NBP2-59343]

Western Blot: TRAP1 Antibody (3H4-2H6) [NBP2-59343] - Analysis of Human, Rat Human A431 and Rat Brain Membrane cell lysates showing detection of ~75 kDa Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone 3H4-2H6. Lane 1: MW ladder. Lane 2: Human lysate, A431. Lane 3: Rat lysate, Rat Brain Membrane (RBM). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Color Development: TMB solution for 15 min at RT. Predicted/Observed Size: ~75 kDa.
Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (3H4-2H6) [NBP2-59343]

Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (3H4-2H6) [NBP2-59343]

Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (3H4-2H6) [NBP2-59343] - Tissue: Myoblast cell line C2C12 . Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.

Applications for TRAP1 Antibody (3H4-2H6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: TRAP1

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is TRAP1. Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein. Immunofluorescence staining of TRAP1 in PC-3-M cells with this antibody produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using this antibody fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1®s inability to associate with these co-chaperones.

Alternate Names

HSP 75, HSP75TNFR-associated protein 1, HSP90Lheat shock protein 75 kDa, mitochondrial, TNF receptor-associated protein 1, TRAP-1, tumor necrosis factor type 1 receptor associated protein, Tumor necrosis factor type 1 receptor-associated protein

Gene Symbol

TRAP1

Additional TRAP1 Products

Product Documents for TRAP1 Antibody (3H4-2H6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TRAP1 Antibody (3H4-2H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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