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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # JOVI.1

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Human HA1.7 TCR beta chain expressed on transgenic mouse cells.

Specificity

Targets TCRBC1+ cells in a selective manner by recognizing 2 amino acid residues specifically exposed in TCRBC1 but not in TCRBC2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TRBC1 Antibody (JOVI.1) - BSA Free

Flow Cytometry: TRBC1 Antibody (JOVI.1) - BSA Free [NBP2-50355]

Flow Cytometry: TRBC1 Antibody (JOVI.1) - BSA Free [NBP2-50355]

Flow Cytometry: TRBC1 Antibody (JOVI.1) - BSA Free [NBP2-50355] - A surface stain was performed on Jurkat cells NBP2-50355AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Applications for TRBC1 Antibody (JOVI.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

reported in scientific literature (PMID 1284120)

Immunoprecipitation

1:10 - 1:500
Application Notes
This antibody is Cytof ready. Use in Immunohistochemistry on frozen sections fixed in acetone reported in scientific literature (PMID: 1284120)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRBC1

Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to be located in plasma membrane. Predicted to be part of immunoglobulin complex, circulating. Predicted to be active in external side of plasma membrane.

Alternate Names

BV05S1J2.2, TCRBC1, TRBC1 T cell receptor beta constant 1

Gene Symbol

TRBC1

Additional TRBC1 Products

Product Documents for TRBC1 Antibody (JOVI.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRBC1 Antibody (JOVI.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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