TRERF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-81654

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all TRERF1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 350 and 400 of human transcriptional regulating protein 132 (transcriptional regulating factor 1) using the numbering given in entry NP_277037.1 (GeneID 55809).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRERF1 Antibody

Immunoprecipitation: TRERF1 Antibody [NB100-81654]

Immunoprecipitation: TRERF1 Antibody [NB100-81654]

Immunoprecipitation: TRERF1 Antibody [NB100-81654] - Detection of human TRERF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRERF1 antibody NB100-81654 used for IP at 3 ug per reaction. TRERF1 was also immunoprecipitated by rabbit anti-TRERF1 antibody NB100-81655. For blotting immunoprecipitated TRERF1, NB100-81655 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for TRERF1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
This antibody can be used for Co-IP application (PMID: 21258344). It is not recommended for Western Blot analysis.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRERF1

The transcriptional regulating protein of 132 kDa (TReP-132/TRERF1) was identified as a transcription factor that interacts with and activates the promoter of the CYP11A1 gene, a gene that encodes cytochrome P450 side chain cleavage (P450scc) and catalyzes the first step in steroidgenesis. TRep-132 can function as a nuclear receptor co-activator and has been shown to interact with Sp1 and the progesterone receptor (PR) to suppress growth via activation of the gene promoters of the cyclin-dependent kinase inhibitors, p21 and p27.

Alternate Names

BCAR2, Breast cancer estrogen resistance 2RAPAHSA277276, dJ139D8.5, FLJ21198, rapa-1, rapa-2, RP1-139D8.5, transcriptional regulating factor 1, transcriptional regulating protein 132, transcriptional-regulating factor 1, Transcriptional-regulating protein 132, TREP132, TReP-132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132

Entrez Gene IDs

55809 (Human)

Gene Symbol

TRERF1

UniProt

Q96PN7 (Human)

Additional TRERF1 Products

Product Documents for TRERF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TRERF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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