TRIM Antibody (TRIM-04) [Allophycocyanin/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-485APCCY7

Novus Biologicals, part of Bio-Techne
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NB500-485APCCY7
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NB500-485
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # TRIM-04

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all TRIM Antibodies »

Product Specifications

Immunogen

Recombinant fragment, corresponding to amino acids 29-186 of Human TRIM.

Specificity

The antibody TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for TRIM Antibody (TRIM-04) [Allophycocyanin/Cy7]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: TRIM

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit alpha after phosphorylation. TRIM has also been shown to associate with TCR alpha, beta, and zeta. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.

Long Name

T Cell Receptor Interacting Molecule

Alternate Names

TCRIM, TRAT1

Gene Symbol

TRAT1

Additional TRIM Products

Product Documents for TRIM Antibody (TRIM-04) [Allophycocyanin/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM Antibody (TRIM-04) [Allophycocyanin/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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