TRIM Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89660

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89660
NBP1-89660-25ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all TRIM Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RQDKMYSYSSDHTRVDEYYIEDTPIYGNLDDMISEPMDENCYEQMKARPEKSVNKMQEATPSAQATNETQMCYASLDHSVKGKRRKPRKQNTHFSDKDGDEQLHAI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM Antibody

Western Blot: TRIM Antibody [NBP1-89660]

Western Blot: TRIM Antibody [NBP1-89660]

Western Blot: TRIM Antibody [NBP1-89660] - Analysis in control (vector only transfected HEK293T lysate) and TRAT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: TRIM Antibody [NBP1-89660]

Immunohistochemistry-Paraffin: TRIM Antibody [NBP1-89660]

Immunohistochemistry-Paraffin: TRIM Antibody [NBP1-89660] - Staining in human lymph node and cerebral cortex tissues using anti-TRAT1 antibody. Corresponding TRAT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry: TRIM Antibody [NBP1-89660]

Immunohistochemistry: TRIM Antibody [NBP1-89660]

Immunohistochemistry: TRIM Antibody [NBP1-89660] - Staining of human tonsil shows cytoplasmic positivity in lymphoid cells outside the reaction centra.
View 2 more images

Applications for TRIM Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit alpha after phosphorylation. TRIM has also been shown to associate with TCR alpha, beta, and zeta. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.

Long Name

T Cell Receptor Interacting Molecule

Alternate Names

TCRIM, TRAT1

Gene Symbol

TRAT1

Additional TRIM Products

Product Documents for TRIM Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for TRIM Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.